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Sharma, Shailesh Assemblies of Wheat EST Sequences and Annotation of Affymetrix Consensus Sequences of Wheat Transcriptome. American Journal of Bioinformatics Research, 5 (1). pp. 16-19. ISSN 2167-6992

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Wheat is an important crop in the world, having the most challenging genomes. Triticum aestivum commonly known as bread wheat is a hexaploid, with three complete genomes termed as A, B and D in the nucleus of each cell. Each of these genomes is almost twice of the human genome and consists of around 6,000 million letters. Presently complete wheat genome sequence is not present. For this reason wheat genome research groups are using different genome assembly approaches and tools. In this paper, we report assembly of wheat ESTs by two different freewares CAP3 [1] and Trinity [2]. The second is an assembly of 61115 Affymetrix consensus sequences representing 42 chromosomes of bread wheat with EST sequences for increasing their length and annotation on the basis of sequence similarity with A, B and D gene models. We report the assembled contig sequences by two different freewares CAP3 [1] and Trinity [2] with their address in the genome which was a lacking information earlier. Trinity [2] was found in the better then CAP3 [1] program because Trinity contigs in comparison to the CAP3 contigs are mapped over gene models much better.

Item Type: Article
Uncontrolled Keywords: EST, Triticum aestivum, Python, CAP3, Trinity
Subjects: Agri-Biotechnology
Depositing User: Dr. Author @NABI
Date Deposited: 12 Mar 2021 04:47
Last Modified: 12 Mar 2021 04:47
URI: http://nabi.sciencecentral.in/id/eprint/46

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