Singh, Sudhir P and Jeet, Raja and Kumar, Jitendra and Vishnu , Shukla and Srivastava, Rakesh and Mantri, Shrikant S and Tuli, Rakesh (2014) Comparative Transcriptional Profiling of Two Wheat Genotypes, with Contrasting Levels of Minerals in Grains, Shows Expression Differences during Grain Filling. PlOS ONE, 9 (11). e111718. ISSN 1932-6203

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Abstract

Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of ≥2 at p≤0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts.

Item Type:	Article
Uncontrolled Keywords:	Wheat,Membrane proteins,Pectins,RNA extraction,Homeostasis,Microarrays,Gene regulation,Meta-analysis
Subjects:	Agri-Biotechnology
Depositing User:	Dr. Author @NABI
Date Deposited:	14 Jul 2015 06:56
Last Modified:	14 Jul 2015 06:56
URI:	http://nabi.sciencecentral.in/id/eprint/26

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