Gupta, Priya and Idris, Asif and Mantri, Shrikant and Asif, Mehar Hasan and Yadav, Hemant Kumar and Roy, Joy Kumar and Tuli, Rakesh and Mohanty, Chandra Sekhar and Sawant, Samir Vishwanath (2012) Discovery and use of single nucleotide polymorphic (SNP) markers in Jatropha curcas L. Molecular Breeding, 30 (3). pp. 1325-1335. ISSN 1572-9788

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Abstract

Various programs for genetic improvement in oil yield of the biofuel plant Jatropha curcas L. are currently in progress worldwide. In order to develop strategies for genetic improvement, it is important to estimate the degree of diversity at the genetic level among various genotypes of J. curcas. High-throughput sequencing of complexity-reduced nuclear genomic DNA of J. curcas coupled with computational analysis discovered 2,482 informative single nucleotide polymorphisms (SNPs). Genotyping of selective SNPs among 148 global collections of J. curcas lines and further diversity analysis through NTSYS-pc, DARwin and Structure 2.0 software revealed that a narrow level of genetic diversity existed among the indigenous genotypes as compared to the exotic genotypes of J. curcas. The level of marker informativeness along with distance-based and Bayesian clustering revealed grouping of the accession from Togo (Africa) with various Indian accessions at K = 4 and K = 5 values (where K represents the number of populations). The diverse accessions identified in the study will be of further use in genetic improvement of J. curcas through quantitative trait loci and association mapping.

Item Type:	Article
Uncontrolled Keywords:	Jatropha curcas Molecular marker Single nucleotide polymorphism High-throughput sequencing
Subjects:	Agri-Biotechnology
Depositing User:	Dr. Author @NABI
Date Deposited:	29 Dec 2015 04:53
Last Modified:	29 Dec 2015 04:53
URI:	http://nabi.sciencecentral.in/id/eprint/31

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