Bhati, Kaushal Kumar and Aggarwal, Sipla and Sharma, Shivani and Mantri, Shrikant and Singh, Sudhir P. and Bhalla, Sherry and Kaur, Jagdeep and Tiwari, Siddharth and Roy, Joy K. and Tuli, Rakesh and Pandey, Ajay K. (2014) Differential expression of structural genes for the late phase of phytic acid biosynthesis in developing seeds of wheat (Triticum aestivum L.). Plant Science, 224. pp. 74-85. ISSN 0168-9452

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Abstract

In cereals, phytic acid (PA) or inositol hexakisphosphate (IP6) is a well-known phosphate storage compound as well as major chelator of important micronutrients (iron, zinc, calcium, etc.). Genes involved in the late phases of PA biosynthesis pathway are known in crops like maize, soybeans and barley but none have been reported from wheat. Our in silico analysis identified six wheat genes that might be involved in the biosynthesis of inositol phosphates. Four of the genes were inositol tetraphosphate kinases (TaITPK1, TaITPK2, TaITPK3, and TaITPK4), and the other two genes encode for inositol triphosphate kinase (TaIPK2) and inositol pentakisphosphate kinase (TaIPK1). Additionally, we identified a homolog of Zmlpa-1, an ABCC subclass multidrug resistance-associated transporter protein (TaMRP3) that is putatively involved in PA transport. Analyses of the mRNA expression levels of these seven genes showed that they are differentially expressed during seed development, and that some are preferentially expressed in aleurone tissue. These results suggest selective roles during PA biosynthesis, and that both lipid-independent and -dependent pathways are active in developing wheat grains. TaIPK1 and TaMRP3 were able to complement the yeast ScΔipk1 and ScΔycf1 mutants, respectively, providing evidence that the wheat genes have the expected biochemical functions. This is the first comprehensive study of the wheat genes involved in the late phase of PA biosynthesis. Knowledge generated from these studies could be utilized to develop strategies for generating low phyate wheat.

Item Type:	Article
Uncontrolled Keywords:	Triticum aestivum; Phytic acid pathway; Seed development; Gene expression; Inositol phosphate kinases; Multidrug resistance protein
Subjects:	Agri-Biotechnology
Depositing User:	Dr. Author @NABI
Date Deposited:	29 Dec 2015 07:30
Last Modified:	29 Dec 2015 07:30
URI:	http://nabi.sciencecentral.in/id/eprint/32

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