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Kumar, J. and Gunapati, S. and Kumar, J. (2014) Virus-induced gene silencing using a modified betasatellite: a potential candidate for functional genomics of crops. Archives of Virology, 159 (8). pp. 2109-2113.

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Betasatellites are geminivirus-associated single-stranded DNA molecules that play an important role in symptom modulation. A VIGS vector was developed by modifying cotton leaf curl Multan betasatellite (CLCuMB). CLCuMB DNA was modified by replacing the beta C1 gene with a multiple cloning site. The silencing ability of the modified CLCuMB was investigated by cloning a fragment of a host gene (Su) or a reporter transgene (uidA) into the modified CLCuMB and co-agroinoculation with cotton leaf curl Multan virus, cotton leaf curl Kokhran virus, and ageratum enation virus, separately. The inoculated Nicotiana tabacum, N. benthamiana, Solanum lycopersicum, Arabidopsis thaliana and Gossypium hirsutum plants showed efficient silencing of the cognate genes.

Item Type: Article
Subjects: Agri-Biotechnology
Depositing User: Unnamed user with email alok@urdip.res.in
Date Deposited: 27 Mar 2015 08:57
Last Modified: 27 Mar 2015 08:57
URI: http://nabi.sciencecentral.in/id/eprint/4

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