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Garg, M. and Yanaka, M. and Mikiko, T. and Tanaka, H. (2014) Introgression of useful genes from Thinopyrum intermedium to wheat for improvement of bread-making quality. Plant Breeding, 133 (3). pp. 327-334.

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To study the influence of genes from Thinopyrum intermedium on traits affecting the bread-making quality of wheat, two derivatives from a putative disomic addition line in cultivar Vilmorin 27' were used in cytological, biochemical and molecular characterization. Cytological analysis suggested that one of the derivatives (Line-1) had a terminal deletion involving the long arm of chromosome 1D (2n=42, Del-1DL), and the other (Line-2) was a conventional addition line, but also carried the same deletion on chromosome 1D (2n=44, Thi+Del-1DL). Amplification and sequencing of high-molecular-weight glutenin subunit (HMW-GS) genes coded by the Th.intermedium chromosome in Line-2 indicated the presence of one x-type with an extra cysteine and four (rather than one) unique y-type genes. Rheological studies of Line-1 showed significantly lower dough strength compared to Vilmorin 27', confirming the recognized role of Glu-1D coded HMW-GSs. Line-2 showed significantly higher dough strength compared to the background cultivar, indicating a significant potential of Th.intermedium for improvement of bread-making quality in wheat.

Item Type: Article
Subjects: Agri-Biotechnology
Depositing User: Unnamed user with email alok@urdip.res.in
Date Deposited: 27 Mar 2015 09:02
Last Modified: 27 Mar 2015 09:02
URI: http://nabi.sciencecentral.in/id/eprint/5

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